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Digation independent cloning serial cloner11/13/2023 ![]() Inventor Gregory Lohman Vladimir Potapov John M. PRYOR Rebecca Kucera Original Assignee New England Biolabs, Inc. The benefits of Clone Digger are: Variety of handled clone types. Inserts are usually PCR amplified and vectors are made linear either by restriction enzyme digestion or by PCR. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and inefficient enzymatic steps. ![]() Priority date (The priority date is an assumption and is not a legal conclusion. Ligation Independent Cloning (LIC) is a technique developed in the early 1990s as an alternative to restriction enzyme/ligase cloning. The precise assembly of specific DNA sequences is a critical technique in molecular biology. 239000011780 sodium chloride Substances 0.000 claims abstract description 26.239000000203 mixture Substances 0.000 claims abstract description 37.108091007521 restriction endonucleases Proteins 0.000 claims abstract description 79.102000003960 Ligases Human genes 0.000 claims abstract description 89.108090000364 Ligases Proteins 0.000 claims abstract description 89.239000002157 polynucleotide Substances 0.000 claims abstract description 203.ΔΆ29920000023 polynucleotide Polymers 0.000 claims abstract description 203.229920003013 deoxyribonucleic acid Polymers 0.000 title claims description 72.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority claimed from US201862747874P external-priority Application filed by New England Biolabs, Inc.
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